Initial results of application of in-vitro fertilization in Hue University Hospital

Abstract

Introduction: Hue Center for Reproductive Endocrinology and Infertility (HUECREI), Hue University Hospital was established in 2003 to meet the demand of infertility treatment for people in Central Region and Central Highlands Vietnam. Our staff have been being trained on assisted reproduction techniques art for many years inside Vietnam and abroad. Since 2010, The Hospital Board of Directors has decided to develop in-vitro fertilization (IVF) and ART in order to improve the quality of treatment. After 2 years of preparation, and assessed through the hospital expertise council, we announced and recruit patients for IVF treatment since 9/2013 and did ovum-pick-up, embryo transfer from 12/2013 and was officially the 18th ART Center in Vietnam. Professional process: carried out in 2012, including issues related to administration, database management, procurement of equipment and machinery, tools and consumables, experimental testing and control the entire process, announce and recruit patients. physical examination, assessment of reproductive function, diagnosis, and conduct IVF for all cases correctly indicated. Ovarian stimulation with different protocols individually, trigger with hCG or GnRHa, ovum-pick-up with vaginal ultrasound, sperm collected from ejaculation or freeze sample or surgery, culture 2-3 days before transfer, embryo transfer with abdominal or vaginal ultrasound, the remaining embryos are frozen by vitrification method. Treatment results: From 12/2013 to 4/2014 a total of 39 couples with 41 IVF cycles recruited. Couples who had previous ivf treatment failure (20.5%), female mean age (34.6 ± 4.1), infertility duration 5.2 ± 2,9 years, scattered from different provinces, with the causes of infertility from both husband and wife. Indication of ovarian stimulation protocols individually include super long, long protocol, short protocol and gnrhantagonist protocol (accounting for 83%). Conventional IVF or ICSI were carried out. The average FSH dose was 2143IU and the average number of follicles from 14mm was 10.5 ± 3.7, trigger mainly with hCG (90.2%), the remaining 9.8% used GnRHa because the risk of ovarian hyperstimulation. Average number of MII oocyte obtained was 9.46 ± 5.7 and the average number of good embryos on day 2 or 3 was 5.7 ± 2.9 per patient, the average embryos number transferred 2.61 ± 0,75. Results of biochemical pregnancy rate per patient was 35.9% and the clinical pregnancy rate was 28.2%. Only one case gets multiple pregnancies (twins) accounted for 7.1% and no one with severe OHSS. Pregnancy rate from cryopreserved sperm was 75%, from surgical sperm 50% and was 100% in ovum recipients, pregnancy rates after thawed embryo transfer was 66.7%. Conclusion: Hue Center for Reproductive Endocrinology and Infertility (HUECREI), after more than 10 years of operation, has successfully and safely application of IVF technique with local human resources, trained from the major ART centers in and outside Vietnam, carried out most of the important assisted reproduction techniques, treated most of the infertility causes with success rates comparable to other ART centers.