In vitro maturation in women with and without polycystic ovarian syndrome

Abstract

Objective: To compare the IVM and pregnancy outcomes between PCOS and non-PCOS patients.

Patients and Methods: A retrospective cohort study was conducted at IVFMD, My Duc Hospital from April 2014 to Jan 2016. A total of 346 IVM cycles were included in the study. Patients were divided into 2 groups, group 1 included 314 PCOS-IVM cycles and group 2 included 32 non-PCOS cycles but having history of OHSS with standard COS. Patients had 3 days of FSH (100 IU/day) and 10000 IU of hCG priming. Oocyte collection was done at 36-38 hours after hCG injection. OCC (oocyte-cumulus complex) were cultured in LAG medium (Origio, Denmark) for 2 hours and cultured in IVM medium supplemented with patient’s serum, hCG, GH and FSH (Origio, Denmark) for 20 hours subsequently. Oocyte maturation was checked at collection, 22 and 26 hours post collection. Patients had day-2 embryo transfer.

Results: There was no difference in patient characteristics between 2 groups (age, BMI, AMH, endometrium thickness and type of fertility). The number of oocytes retrieved is higher in PCOS patients (13.00 vs 11.00, p=0.005). There was no difference in the number of matured oocytes (8.00 vs 6.00, p=0.103), fertilized oocytes (5.00 vs 5.00, p=0.473), day-2 embryos (5.00 vs 4.00, p=0.548) and the top-quality embryos (1.00 vs 1.00, p=0.462) between PCOS and non-PCOS patients. There was no difference in number of embryos transferred (3.0 vs 3.0), clinical pregnancy rate (48.7% vs 46.9%), ongoing pregnancy rate (36.0% vs 31.2%), implantation rate (23.8% vs 25.9%) (p>0.05)

Conclusion: IVM for non-PCOS patients does not show any inferior outcomes compared to PCOS patients.